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Literature summary extracted from

  • Cardinale, G.J.; Abeles, R.H.
    Purification and mechanism of action of proline racemase (1968), Biochemistry, 7, 3970-3978.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.1.4 2-mercaptoethanol activates Acetoanaerobium sticklandii
5.1.1.4 2-mercaptoethanol optimal concentration: 0.02 M Acetoanaerobium sticklandii
5.1.1.4 additional information addition of pyridoxal 5'-phosphate does not increase activity Acetoanaerobium sticklandii
5.1.1.4 NaBH4 activates Acetoanaerobium sticklandii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.4 1,10-phenanthroline
-
 Acetoanaerobium sticklandii
5.1.1.4 2-Furoic acid weak Acetoanaerobium sticklandii
5.1.1.4 2-Thiophenecarboxylic acid
-
 Acetoanaerobium sticklandii
5.1.1.4 alpha-Pipecoline
-
 Acetoanaerobium sticklandii
5.1.1.4 Asp
-
 Acetoanaerobium sticklandii
5.1.1.4 Fumaric acid
-
 Acetoanaerobium sticklandii
5.1.1.4 HPO42- no inhibition by H2PO4- Acetoanaerobium sticklandii
5.1.1.4 iodoacetamide
-
 Acetoanaerobium sticklandii
5.1.1.4 iodoacetate DL-Pro and pyrrole-2-carboxylate can protect Acetoanaerobium sticklandii
5.1.1.4 Maleic acid
-
 Acetoanaerobium sticklandii
5.1.1.4 malonic acid
-
 Acetoanaerobium sticklandii
5.1.1.4 Methoxyacetic acid weak Acetoanaerobium sticklandii
5.1.1.4 Phthalic acid
-
 Acetoanaerobium sticklandii
5.1.1.4 pipecolic acid weak Acetoanaerobium sticklandii
5.1.1.4 pyrrole-2-carboxylic acid
-
 Acetoanaerobium sticklandii
5.1.1.4 sarcosine
-
 Acetoanaerobium sticklandii
5.1.1.4 succinic acid
-
 Acetoanaerobium sticklandii
5.1.1.4 Tetrahydrofuroic acid weak Acetoanaerobium sticklandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.4 2.3
-
 L-Pro
-
 Acetoanaerobium sticklandii
5.1.1.4 3.8
-
 D-Pro
-
 Acetoanaerobium sticklandii

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.4 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.4
-
 Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.4 600
-
-
 Acetoanaerobium sticklandii

Storage Stability

EC Number Storage Stability Organism
5.1.1.4 stored 6 months in the refrigerator without significant loss of activity Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.4 L-Pro
-
 Acetoanaerobium sticklandii D-Pro
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.4 70
-
 half-life: 3.6 min, in D2O 17 min Acetoanaerobium sticklandii